CIBSS - Centre for integrative Biological Signalling StudiesCIBSS

Associate Investigators

Prof. Dr. Alexander Rohrbach

Prof. Dr. Alexander Rohrbach

Contact

Prof. Dr. Alexander Rohrbach
IMTEK Department of Microsystems Engineering (Faculty of Engineering)
University of Freiburg

T +49 761 203 7536
rohrbach(at)imtek.de

Further Information

Living cells are fascinating microsystems driven by a variety of physics principles. For instance, it is well known thatnearly every transport and interaction process in living cells is governed by thermal noise. However, it is hardly known or often disregarded that while interactions can be visible on one timescale, they can be completely invisible on another. Therefore, it is not only necessary to measure on a broad frequency range, but also to decompose the broadband fluctuation data with appropriate models. Therefore, we develop fast optical measurement technology and biophysical computer simulations to understand biological interactions on time scales from minutes to micro-seconds – at or beyond the limits of diffraction, of photon noise and of diffusion.

We advance novel super-resolving laser optical microscopy with and without labels and we advance optical tweezers-based applications such as Photonic Force Microscopy, with MHz interferometric tracking.  With this we investigate the biophysics of living immune cells, of bacteria and of bio-mimetic systems, where we record and analyze the nano-mechanics and thermal fluctuations of cellular structures and their interaction partners. These approaches will help us to better understand e.g. infection diseases.

 

Keywords

Molecular interaction, Across time scales, Broad bandwidth measurements, Super-resolution microscopy, Optical tweezers, 3D particle tracking, Computer simulations, Particle binding to cells, Phagocytosis, Bacterial cytoskeleton and cell wall



Thermal noise drives cellular structures and particles on nanometer and microsecond scales. This motion regulates the life of each cell and organism. If we want to understand this motion, we have to measure it.

10 selected publications:

  • Fast, label-free super-resolution live-cell imaging using rotating coherent scattering (ROCS) microscopy
    Jünger F, Olshausen P, Rohrbach A (2016).
    Sci Rep. 28;6:30393
  • Separation of ballistic and diffusive fluorescence photons in confocal Light-Sheet Microscopy of Arabidopsis roots.
    Meinert T, Tietz O, Palme KJ, Rohrbach A (2016).
    Sci Rep. 6:30378.
  • Surface imaging beyond the diffraction limit with optically trapped spheres.
    Friedrich L, Rohrbach A (2015).
    Nat Nanotechnol. 10(12):1064-9.
  • Measuring local viscosities near plasma membranes of living cells with photonic force microscopy.
    Jünger F, Kohler F, Meinel A, Meyer T, Nitschke R, Erhard B, Rohrbach A (2015).
    Biophys J. 109(5):869-82.
  • Superresolution Imaging of Dynamic MreB Filaments in B. subtilis - A Multiple-Motor-Driven Transport?
    v Olshausen P, Soufo HJD, Graumann P, Wicker K, Heintzmann R, Rohrbach A (2013).
    Biophys J. 105(5):1171 – 1181
  • Object adapted optical trapping and shape tracking of energy switching helical bacteria.
    Koch M. Rohrbach A (2012).
    Nat Photonics, 6, 680 - 686
  • Microfluidic sorting of arbitrary cells with dynamic optical tweezers.
    Landenberger B, Höfemann H, Wadle S, Rohrbach A (2012).
    Lab Chip, 12, 3177-3188.
  • Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media.
    Fahrbach FO, Rohrbach A (2012)
    Nature Communications 3: p. 632.
  • Microscopy with self-reconstructing beams.
    Fahrbach, FOP, Simon P, Rohrbach A (2010). 
    Nature Photonics. 4(11):780-785
  • Filopodia act as phagocytic tentacles and pull with discrete steps and a load-dependent velocity.
    Kress H, Stelzer EHK, Holzer D, Buss F, Griffiths G, Rohrbach A (2007). 
    Proc Nat Acad Sci.104, 11633–11638