CIBSS - Centre for integrative Biological Signalling StudiesCIBSS

Expression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms

Top O, Milferstaedt SWL, van Gessel N, Hoernstein SNW, Özdemir B, Decker EL, Reski R.

Commun Biol. 2021; doi: 10.1038/s42003-021-02486-3.

We report here that the expression of a human CDS in Physcomitrella resulted in various transcript isoforms. Mutating experimentally verified donor and acceptor sites as well as increasing GC% of the cryptic introns prevented undesired transcripts and boosted functional protein production.