Optimized biochemical method for human Polyphosphate quantification

Lázaro B, Sarrias A, Tadeo FJ, Marc Martínez-Láinez J, Fernández A, Quandt E, Depares B, Dürr-Mayer T, Jessen H, Jiménez J, Clotet J, Bru S.

Methods. 2025; doi: 10.1016/j.ymeth.2025.01.001

This paper provides a detailed protocol how to extract reliably polyphosphate from different mammalian cell lines and optimizes extraction efficiency. The protocol is validated by several state of the art methods.