T +49 761 270 63097
olaf.gross(at)uniklinik-freiburg.de
We investigate danger sensing and innate immune signalling in macrophages, exploring their intricate connections with metabolism, cell death, and cellular structures. Macrophages exhibit fascinating cross-talk related to cell death, as they respond to danger signals released during cellular demise and can themselves engage various genetically defined cell death pathways, each with varying inflammatory potential, some of which are even triggered as a consequence of innate immune signalling. We are especially interested in the sensing mechanism of sterile endogenous or environmental danger signals, and in indirect danger sensing such as involving the effects of pathogens on host cell signalling and function. Metabolism and organelles emerge as information-bearing signalling entities in this context. Our primary focus lies on two families of pattern recognition receptors: inflammasomes and ITAM-coupled receptors that both not only respond to pathogens, but also recognize sterile danger signals. Inflammasomes, nucleated by cytoplasmic receptors, control the unconventional release of interleukin-1 family cytokines and trigger a lytic form of cell death called pyroptosis via activity of their constituent protease caspase-1. ITAM-coupled receptors on the cell surface engage NF-κB transcription factors, leading to macrophage activation and the expression of proinflammatory cytokines, as well as providing an essential inflammasome priming signal. Our investigations of the functional consequences of these mechanisms in the context of host defence, homeostasis, immune pathology, development, and cancer extend to various tissues, including the brain, heart, lung, and bone marrow. Ultimately, our research aims to contribute fundamental insights into the molecular mechanisms of innate immunity and inflammation. By identifying rational targets, we hope to steer immunity and develop control-of-function tools, particularly for cancer-related applications.
