CIBSS - Centre for integrative Biological Signalling StudiesCIBSS

CIBSS-D

Bessel light-sheet microscopy: object adapted illumination and opto-genetic switching with computer holograms

Prof. Dr. Alexander Rohrbach (CIBSS-AI), IMTEK (Faculty of Engineering), University of Freiburg

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In this project we will develop a novel light sheet microscope, which is equipped with two spatial light modulators (SLM) for high end 4D imaging of large scattering specimen and precise Opto genetic switching. Unprecedented image quality will be achieved by using holographically shaped, object adapted, confocal Bessel beams for imaging various samples provided by the collaboration partners. By a novel postprocessing method, we are able to separate diffusive and ballistic photons, which leads to high image quality also deep inside the specimen. By developing a feedback controlled, holographic multipoint laser illumination through the second SLM, we will realize dynamic and precise Opto genetic switching in large specimen with superior precision and efficiency.

 

Publications resulting from the project:

100 Hz ROCS microscopy correlated with fluorescence reveals cellular dynamics on different spatiotemporal scales.
Jünger F, Ruh D, Strobel D, Michiels R, Huber D, Brandel A, Madl J, Gavrilov A, Mihlan M, Daller CC, Rog-Zielinska EA, Römer W, Lämmermann T, Rohrbach A.
Nat Commun. 2022; doi: 10.1038/s41467-022-29091-0.

Towards non-blind optical tweezing by finding 3D refractive index changes through off-focus interferometric tracking.
Landenberger B, Yatish, Rohrbach A.
Nat Commun. 2021 Nov 26;12(1):6922. doi: 10.1038/s41467-021-27262-

A Shape-switch-block principle for confocal light-sheet microscopy with sectioned Bessel beams and stimulated emission depletion
Koebele L, Rohrbach A
Commun Phys 2020. doi.org/10.1038/s42005-020-00458-3

Light-sheet microscopy with length-adaptive Bessel beams.
Meinert T, Rohrbach A.
Biomed Opt Express. 2019 Jan 18;10(2):670-681. doi: 10.1364/BOE.10.000670.