Chem Commun (Camb). 55(37):5339-5342
Magic spot nucleotides: tunable target-specific chemoenzymatic synthesis
A tunable chemoenzymatic strategy provides access to the entire class of magic spot nucleotides and modified analogues. The approach combines chemoselective bisphosphorylations using phosphoramidites with regioselective Ribonuclease T2 cyclo-phosphate hydrolysis, leading to flexible and simple gram-scale operations.