CIBSS - Centre for integrative Biological Signalling Studies CIBSS

Rapid phagosome isolation enables unbiased multiomic analysis of human microglial phagosomes

Wogram E, Sümpelmann F, Dong W, Rawat E, Fernandez Maestre I, Fu D, Braswell B, Khalil A, Buescher JM, Mittler , Borner GHH, Vlachos A, Tholen S, Schilling O, Bell GW, Rambold AS, Akhtar A, Schnell O, Beck J, Abu-Remaileh M, Prinz M, Jaenisch R.

Immunity. 2024 Sep 10; doi: 10.1016/j.immuni.2024.07.019.

Here, we developed a method for rapid isolation of pure and intact phagosomes from human pluripotent stem cell-derived microglia under various in vitro conditions, and from human brain biopsies, for unbiased multiomic analysis.